mosquito spt labtech robot  (SPT Labtech)

Journal: Cell Reports Methods

Article Title: An automated platform for structural analysis of membrane proteins through serial crystallography

doi: 10.1016/j.crmeth.2021.100102

Figure Lengend Snippet: In meso crystallization and SSX analysis of membrane proteins in CrystalDirect plates On the left is a CrystalDirect (CD) plate with LCP crystallization experiments (inset). Once LCP crystals have grown, two different workflows for structural analysis can be applied under the same crystallization conditions. Room-temperature in situ X-ray diffraction data can be collected directly from the CrystalDirect plate (middle top). For cryogenic structures (middle bottom), a workflow including automated laser-based harvesting and cryo-cooling can be utilized. Room-temperature and cryogenic structures of ADIPOR2 (right, top and bottom, respectively) obtained with these approaches are shown.

Article Snippet: For soaking experiments, the top sealing film of the CrystalDirect plate was removed and 30–150 nl of Tb-XO4 or 60–120 nl of Gd-DO3 ligand solutions were delivered directly to the LCP crystallization drops with a SPT-Labtech crystallization robot, after which the plate was resealed.

Techniques: Crystallization Assay, Membrane, In Situ

Journal: Cell Reports Methods

Article Title: An automated platform for structural analysis of membrane proteins through serial crystallography

doi: 10.1016/j.crmeth.2021.100102

Figure Lengend Snippet: A pipeline for high-throughput ligand discovery with membrane protein crystals After LCP crystallization in CrystalDirect plates, crystals can be directly accessed by removal of the top plastic seal on the plate (top row). Soaking solutions are introduced into the experiment by a pipetting robot, after which the plate is resealed and incubated for the necessary time (top row). After soaking, LCP crystals are auto - harvested with the CrystalDirect robot into pins and stored in UniPucks for shipment to the synchrotron (middle row, “Experiment”). Serial diffraction data are collected for each uniquely soaked bolus and merged after established protocols before structure refinement and ligand fitting (bottom row, “Results”). The automated data flow in the pipeline, shown by double-arrowed dashed lines, is maintained through the CRIMS, through which users can access, monitor, and analyze data via web interfaces.

Article Snippet: For soaking experiments, the top sealing film of the CrystalDirect plate was removed and 30–150 nl of Tb-XO4 or 60–120 nl of Gd-DO3 ligand solutions were delivered directly to the LCP crystallization drops with a SPT-Labtech crystallization robot, after which the plate was resealed.

Techniques: High Throughput Screening Assay, Membrane, Crystallization Assay, Incubation